Antimicrobial effects of essential oils and their interaction against Listeria monocytogenes

Authors: Vũ Thu Trang*, Vũ Thị Oanh

Abstract

The aim of this study was to evaluate the antimicrobial 8 plant essential oils (EOs): Cinamomum lourerii, Chenopodium ambrosiodes, Litsea cubeba, Zanthoxylum rhetsa, Mentha arvensis, Melaleuca leucadendra, Ocimum gratissium, Psidium guajaua against food-borne bacteria Listeria monocytogenes (L.monocytogenes). The experiment was carried out by the standard disc diffusion method and two-fold serial dilution ELISA. The results indicated that all tested EOs showed the growth inhibition effects with the inhibition zones ranged from 10 mm (Zanthoxylum rhesta) to 75 mm (Litsea cubeba) in diameter against three isolated strains of L.monocytogenes from food. The highest inhibition activities were found in the EOs of Cinamomum lourerii and Litsea cubeba with the inhibition zones ranged from 55-75 mm. The MIC and MBC of Cinamomum lourerii and Litsea cubeba against 3 test strains were 0.39 µl/ml and 0.78 µl/ml, respectively. The synergistic effect in the combination of Cinamomum lourerii and Litsea cubeba enhance 4-8 times of the inhibition effect against L.monocytogenes leading to the reduction of EO dose used in food and in medical espect

Keyword

Essential oils, Listeria monocytogenes, Antimicrobial activity, Synergistic effect
Pages : 145-149

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