Glycosaminoglycans sulphate (GAGs) belonging to a family of proteoglycans are complex natural polysacchrides in animals. Chondroitin sulfate (CS) is a major class of Glycosaminoglycans sulphate used for treatment of arthritis, especial osteoarthritis. Glycosaminoglycans sulphate can be extracted from cartilage of as cows, pigs, chicken, sharks, etc. The extraction of Glycosaminoglycans sulphate from agricultural waste sources for food supplements is meaningful for Vietnam to substitute the exported material. Our research is aimed to determine the optimal condition of hydrolysis of chicken keel cartilage by Alcalase 2.4 L to obtain Glycosaminoglycans sulphate. The optimal values for the hydrolysis process are pH at 7,0; temperature at 540C; the ratio of enzyme and substances at 4 %v/wpro and hydrolysis time of 212 minutes. Meanwhile, Glycosaminoglycans sulphate sulfate content are obtained according to the predictions of the model at 27,78% (dry weight of raw material). Glycosaminoglycans sulphate powder product was found to be Glycosaminoglycans sulphate: 53,61% (dry basis); Chondroitin sulfate determined by HPLC: 37,32% (dry basis).
Keyword
Chondroitin sulfate, Glycosaminoglycan, Alcalase, chicken keel cartilage, hydrosis.
