In this paper we present the results of the production of poliovirus I, II and III for the purification process and the development of inactivated vaccines. The results showed that the ultrafiltration of the poliovirus resulted in the increase of poliovirus density over 80 times; the average recovery yield of the 3 types was 93.54%. After ultracentrifugation the average recovery yield of the 3 types was achieved as 60,10 %. The average recovery yield of the 3 poliovirus types after purification through the column chromatography was 91.11 %. Following purification, the poliovirus was inactivated by formalin and the total of antigen titer was highest for Type III (1.54 x 105 DU) and lowest for Type II (5.36 x 104 DU). The properties of post-refined and inactivated poliovirus suspension meet the requirements for producing the bulk of polio vaccines.
