In this paper we present our research on the development of impedimetric sensor for Chloramphenicol (CAP) detection by using artificial bioreceptor MIP. Our results show that fabricated sensor has high selectivity and low detection limit (LOD = 3,67 nM). Furthermore, the process for sensor fabrication has low cost as cheap screen-printed carbon ink electrode but possesses many advantages of possibility for using Au-printed electrode, simple measurement method, short fabrication time and good reproducibility. Besides, based on current study, it was found that EIS is an impressive method for monitoring the rebinding of target molecules with their surrounding polymer matrix that occurred on the electrode surface. The sensor platform could be easily applied for detection of other small proteins, with selection of the detection platform based on the desired for sensitivity.