Optimizing of conditions for saponin extract process from Carica papaya L. leaves

Authors: Bùi Thị Niên*, Đỗ Thị Hoa Viên


Papaya leaves are used to support for treatment of cancer in some countries such as Australia, American, Brasin, Vietnam… Papaya leaf contains alkaloid, saponin, carotenoid, phenolic compounds… In this study we optimise saponin extracted conditions from papaya leaves by using Design Expert program, and qualitative analyse saponin in papaya leaves. The total extract are extracted by MeOH. Three main factors are examined. There are extracted time, temperature and the ratio of material/extracted solvent. The research result show that optimised extracted conditions for total extract from papaya leaves are extracted time: 52.5 hours, the rate of material/solvent: 1/7 and extracted temperature: 48.51oC. Continue to extract saponin from the total extract with n-butanol, then purify by chloroform, we obtained total crude saponin with the content is 1,69 ± 0,37% compairing with dried material. Qualitative analyse saponins with foaming reaction and thin layer chromatography, we determine that saponins presented in papaya leaves is saponin steroid.


Extract, optimization, Design Expert, saponin, saponin steroid.
Pages : 96-100

Related Articles:

Authors : Lê Minh Tân, Trần Thu Thuỷ, Vũ Duy Đạt, Cao Xuân Trường, Nguyễn Đình Hữu Đức, Trần Thiên Đức*
Authors : Nguyễn Quốc Minh*, Võ Tá Đông, Nguyễn Duy Minh
Authors : Vũ Văn Trọng, Trương Thị Hồng Ngọc, Lê Quân, Vũ Văn Huy, Bùi Thanh Duy, Nguyễn Lê Huy*, Nguyễn Vân Anh, Nguyễn Tuấn Dung
Authors : Lưu Thị Lan Anh*, Nguyễn Ngọc Trung
Authors : Mạc Thị Bích *, Phạm Thị Hoa , Bành Tiến Long , Nguyễn Đức Toàn *
Authors : Nguyễn Ngọc Thắng*, Phạm Thị Ngọc , Vũ Tiến Hiếu, Bùi Văn Huấn