Sulfur-based autotrophic denitrification is an effective treatment technique to remove nitrogen in water and wastewater, in which sulfur compounds act as an electron-donors for the conversion of nitrate to nitrogen gas by autotrophic microorganisms. The main advantage of this process is no external organic carbon source compared with the conventional heterotrophic denitrification process, which resulted in reducing the cost of treatment and the poisoning effect of some organic carbon compounds. In this study, two sulfur-based carriers (C1 and C2) which have different mass ratios with the same core components as elemental sulfur and calcium carbonate but varying adhesive volumes were prepared to evaluate the sulfur-driven denitrification performance. The results show that the nitrate removal rates of C1 and C2 were 0.34 ± 0.04 and 0.32 ± 0.02 kg NO3--N/m3/d, respectively. Beside that, the sulfate concentration generated by autotrophic denitrification were quite high at 273.5 ± 27.8 and 251.3 ± 17.0 mg SO42-/L, respectively.
